Based on AMMI stability value and yield stability index, lines were identified with high yield across locations.Ī seedling-stage drought stress study of 540 S6 Global MAGIC lines was conducted. The environmental effect explained the highest variation, indicating that the environments were diverse, followed by G and G × E effects. The AMMI analysis of variance for yield detected significant effects for genotype, environment, and their interaction (G × E). Yield data were analyzed using the additive main effect and multiplicative interaction model (AMMI) and GGE biplot. Based on yield and agronomic performance in the first MET, 130 lines have been included in a larger MET with sites in the Philippines and Vietnam. Yield data showed considerable transgressive segregation for yield relative to the checks and the founder parents (N. To determine the yield stability and performance of MAGIC lines, an S2:4 bulk population (400 lines) was evaluated under irrigated lowland conditions using a row-column design in a multi-environment trial (MET) with three sites in the Philippines and one site in Tanzania. This new genetic resource promises to be a valuable source of breeding-ready materials for the development of higher-yielding rice varieties.
(NOTE: Hei will provide an Excel document with the results of this genotyping and this could be linked to a Dropbox file or equivalent)įor mapping of new QTLs, the MAGIC population was used to identify resistance to brown spot and bacterial leaf streak diseases.
Preliminary analysis suggests that LD decayed to background levels within 10 cM, indicating high levels of recombination in both populations. These genotypes were used to estimate recombination fractions, linkage disequilibrium, and ancestral haplotypes. The eight parents, 200 S4 Indica MAGIC lines, and 190 MAGIC PLUS S4 lines were genotyped using genotyping by sequencing. Genotyping-by-sequencing (GBS) was applied to populations and approximately 17,000 SNP markers were used for GWAS for multiple traits, including: blast and bacterial blight resistance, salinity and submergence tolerance, and grain quality.
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